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doi:10.1152/physiolgenomics.00064.2001 Castro RE, Ferreira DM, Afonso MB, Borralho PM, Machado MV, Cortez-Pinto H, Rodrigues CM (2013) miR-34a/SIRT1/p53 is suppressed by ursodeoxycholic acid in the rat liver and activated by disease severity in human non-alcoholic fatty liver disease

The resulting homogenate was centrifuged (two min max rpm) and 600 l supernatant was applied to an activated and equilibrated RP18 solid phase extraction column (activation with 1 ml acetonitrile and equilibration with 1 ml methanol/H 2 O [80/20, vol/vol] [Phenomenex Strata C18-E, 55 m, 50 mg/1 ml