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For this purpose, we adopted two approaches: (1) a proteomic approach using FLAG-tagged macrocycles and (2) live-cell imaging using fluorescently labelled macrocycles

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1f), as previously described 34

Notably, the existence of differently sized routes of leak pathway that are regulated by neither different tight junction components nor the degree of decrease of a similar barrier-forming tight junction has been demonstrated

The safety profile and side effect frequency are comparable, though individual experiences vary based on genetics, dose, and body composition