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Ma Y, Zhang Y, Chen J, et al

Measurement of oxidized glutathione and reduced glutathione To measure GSH, 10L of saliva were incubated with 10L of a solution of ethanol o-phthaldehyde (OPT, 1mg/mL) and 180L of phosphate buffer (sodium phosphate 100mM, 2.5mM EDTA-Na2, pH 8.0) for 15min at room temperature

Editing mechanisms in aaRSs Despite the high affinity of aaRSs for their substrates, noncognate amino acids are sometimes activated and charged onto tRNAs, producing misacylated tRNAs that may, upon reaching the ribosome, be used in protein synthesis

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Allele frequencies for genetic polymorphism are such that ~1 in 300 Caucasians is homozygous for a defective allele or alleles for the trait of very low activity, ~11% of people are heterozygous and have intermediate activity