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The ketogenic diet could play a role in SIRT1 activity through increased NAD + , which is a required substrate for SIRT1 (Maalouf et al., 2007

I used S-acetyl-glutathione instead, a stabilized form that resists oxidation and stays intact until your body can actually use it. I also added the cofactors glutathione depends on to work: selenium, riboflavin, and molybdenum

doi: 10.1111/j.1365-2109.2009.02462.x 14 LiL.LimbuS

Front Immunol (2022) 13:816282

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Protein bands were detected using an enhanced chemiluminescence western blot detection system (WesternBright TM ECL-spray from Advansta )