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Meanwhile, 1 ml of slurry of Dynabeads MyOne Streptavidin C1 (Invitrogen, 65002) was washed according to the manufacturers instructions, then pre-bound to 1 nmol of gel-purified biotinylated, 2 O -methylated capture oligonucleotide containing an 8mer seed-match site with complementarity to the loaded miRNA (Supplementary Table 4), at 25 C for 30 min with end-over-end rotation, followed by four washes with ice-cold equilibration buffer (18 mM HEPES pH 7.4, 100 mM NaCl, 1 mM MgCl 2 , 10% (v/v) glycerol, 0.003% (v/v) IGEPAL CA-630, 0.033 mg ml 1 recombinant albumin (NEB, B9200) and 0.005 mg ml 1 yeast transfer RNA (tRNA) (Life Technologies, AM7119)), then set aside at 4 C until use

Although -oxidation is impaired in the setting of VPA toxicity, this metabolite has a prolonged elimination half-life and could be responsible for the prolonged coma that is sometimes observed despite the normalization in plasma VPA concentrations [17,18]

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