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mouse anti--actin, dilution 1:1000) were added for overnight incubation at 4 C and the antibody-bound proteins were developed with the enhanced chemiluminescence reagent coupled with horseradish peroxidase-conjugated secondary antibody

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Therefore, it is thought that HERC2 deficiency would result in a malfunctioning response to elevated iron levels, resulting in ferritinophagy, free iron release, and neuronal cell damage

doi: 10.1111/cns.13207, 72

Iron homeostasis and disorders revisited in the sepsis