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This includes disorganization of the annular lamellae with decreased proteoglycan content, bifurcation, and interdigitation (Lai et al., 2015)

[324] The Emergency Care Access & Timeliness eCQM therefore provides an alternative approach to quality measurement used to address ED boarding and barriers to emergency care by capturing multiple components of quality and capacity

Electron microscopy sample preparation and data acquisition A 3Ll volume of 5LmgLml 1 purified SLC33A1 MAP was mixed with Pmab1 Fv-clasp at a 1:1.5 molar ratio and 20mM GSSG, incubated for 30 min on ice and then applied to glow-discharged Au 400 mesh QUANTIFOIL R1.2/1.3 holey carbon grids (Quantifoil) or Graphene Oxide Au 400 mesh QUANTIFOIL R1.2/1.3 grids before being plunged into liquid-nitrogen-cooled liquid ethane using an FEI Vitrobot Mark IV (FEI Thermo Fisher)

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A recurring technical pitfall is that GSH oxidizes rapidly to GSSG during sample handling, so studies that fail to properly stabilize and derivatize samples can artificially inflate the apparent oxidized fraction a source of noise across the literature