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J Diet Suppl 21(2):210241 Zin MT, Kaewkod T, Chaipoot S, Kanthakat G, Chen Y-Y, Cheirsilp B et al (2025) Ultrasound-assisted deep eutectic solvent extraction of antioxidant and anti-colorectal cancer proteins from Spirulina biomass: process intensification, characterization, and bioactivity evaluation

Disulfides were reduced with 4 mM dithiothreitol (DTT) at RT for 1 h and then alkylated with 10 mM indole-3-acetic acid (IAA) for 15 min in the dark

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Cell Physiol Biochem 29:543550

Pause, assess, recalculate, and resume only when the cause is identified

Evening application creates better alignment between when the peptides arrive at target tissues and when those tissues are most receptive to regenerative signals