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Biochem 12, 149151 [DOI] [PubMed] [Google Scholar] 59.Temel Y et al

The significance of the additional FBXO22-NSD2 interaction surface, which is not interacting with the compound, was further assessed in a proximity-based Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) assay which uses a biotinylated version of the FBXO22 FIST domain and His-tagged NSD2 PWWP1