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Livers were homogenized in 10mL of cold 0.1% IGEPAL and spleens were homogenized in 5mL using a Tissue Tearor Model 985370 (Biospec Products) at 10,000 RPM for 5 seconds/organ

It is also a primary site of drug detoxification, which relies heavily on GSH availability 28

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The rd1 mouse is a common animal model used to study RP in which the expression of over 60 genes, related to proliferation, apoptosis, and transcription, is modified via a mutation in the phosphodiesterase-6 gene, which affects the metabolism of cyclic guanosine monophosphate

coli , this system includes the disulfide isomerase DsbC/G and the reductive power from DsbD, which itself is coupled to the cytoplasmic NADPH pool through Thioredoxin A (TrxA) (Andersen et al., 1997