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JH-EsoAd1 cells were transduced with FUCas9 and sorted for mCherry-positive cells followed by transduction with FGT1-UTG and sorted for GFP-positive cells

In sickle cell anemia oxidative stress can trigger eryptosis and the release of red blood cell microparticles (RBC-MPs), which are small phospholipid-containing vesicles implicated in physiological processes such as hemostasis and potentially linked to macrovascular dysfunction

HEK293T cells at roughly 60% confluency were infected with crude lentivirus followed by selection with 10 g ml 1 puromycin for 1 week