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On the experimental day, cells were washed twice and placed into KRB buffer (132.2 mM NaCl, 3.6 mM KCl, 5 mM NaHCO 3 , 0.5 mM NaH 2 PO 4 , 0.5 mM MgCl 2 , 1.5 mM CaCl 2 and 0.001 g/mL BSA, pH 7.4) supplemented with 25 mM glucose (1 h, 37 C, 5% CO 2 )

Overall, our results establish [ 18 F]FSPG as a predictive marker of therapy resistance in NSCLC and provide the basis for the clinical evaluation of both imaging and therapeutic agents that target this important antioxidant pathway

Nevertheless, its effectiveness remains limited by the intestinal mucosa's low permeability, which restricts drug absorption and overall bioavailability