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Description

Messenger RNA was purified using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) following manufacturers protocol, scaled up to 100 g of total RNA input per sample

Using cryogenic electron microscopy (cryo-EM), we determine the structures of human MRP2 in three conformational states: an autoinhibited state, a substrate-bound pre-translocation state, and an ATP-bound post-translocation state