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US, 6 Johns Hopkins University, 7 Indiana University School of Medicine

Mix 1, containing 1900 L of Buffer X (Mitenyi Biotec, #130-092-628), 50 L of Papain (Mitenyi Biotec, #130-092-628), and 20 L of collagenase (Sigma-Aldrich, #C2139) prepared at a concentration of 100 mg/mL in DMEM-12, was added to the tissue preparation and incubated for 15 min at low temperature

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These functions are regulated by the intrinsic electrical and mechanical properties of smooth muscle, through tonic contractions that maintain organ dimensions against luminal content (e.g., food bolus) and/or through the development of contractile force and muscle shortening (Bitar, 2003)

438,439 With the recognition of metabolic reprogramming inherent in disease progression, particularly in malignancies, it is becoming essential to explore the value of implementing dietary interventions and translating the evidence into practice

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