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The enzyme assays were carried out at 37 C for 1 h in a total volume of 25 l and the assay mixtures contained 50 mM Tris buffer pH 8.0 with 4 mM glutamate, 50 mM NaCl, 50 mM NH 4 Cl, 10 mM ATP and 50 mM MgCl 2 and 0.51.0 g of recombinant protein

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Immunization with hydatid cyst wall antigens can inhibit breast cancer through changes in serum levels of Th1/Th2 cytokines

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These lead to reduced complex I activity and impaired mitochondrial calcium uptake [40]