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Metabolic shift in hypoxia-adapted CML cells and mechanisms of acquired dasatinib resistance uncovered by proteomic iTRAQ profiling

They tested two doses: 10 g/kg and 10 ng/kg (that is a thousand times less!)

These metabolites are absorbed in the small intestine, bind to serum albumin, and are transported to the liver, where they undergo sulfation, glucuronidation, and methylation to form stable metabolites, which are then distributed throughout the body (Figure 3) (Manach et al., 1995

Extracted proteins were quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific #23225)

[DOI] [PubMed] [Google Scholar] 112.Wolf J et al

Results vary, but many notice increased energy and sharper focus within days