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Isolation of mRNA and RTqPCR analysis RNA was extracted from organoid cultures or freshly isolated tissue using the RNeasy Mini RNA Extraction Kit (Qiagen) with DNase treatment and reverse-transcribed using Moloney murine leukaemia virus reverse transcriptase (Promega)

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Immunomonitoring in 25 assessable patients indicates that 84% demonstrate at Least a two-fold enhancement in immune responses to one or more of the six vaccine-encoded tumor Antigens, including 40% with Functional T-cell responses And 80% with elevated antigen-specific antibody levels

These results indicate that inflammation-related proteins tended to be upregulated in the circulation after MMR vaccination, although the effect size was limited

Wong VG, Lietman PS, Seegmiller JE (1967) Alterations of pigment epithelium in cystinosis

(2) Left-sided giant CDH