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Description

5,6-Epoxy-cholestan-3-ol (cholesterol -oxide): a specific substrate for rat liver glutathione transferase B

doi: 10.1111/j.1523-1755.2004.00797.x

The transporters for cystine and cysteine are also dysregulated in HD (Li et al ., 2010

Western blotting Cells were lysed in lysis buffer (20 mM Tris, 150 mM NaCl, 1% Nonidet P-40, 1 mM EDTA, 1 mM EGTA, 10% glycerol, and protease and phosphatase inhibitors), incubated on ice for 15 min, and centrifuged at 16,000 x g for 10 min at 4 C