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Effects of red blood cell (RBC) transfusion on sickle cell disease recipient plasma and RBC metabolism

Cell lines were maintained at 37 C in a humidified incubator with 5% CO 2 and routinely tested for mycoplasma using MycoAlert Kit (Lonza)

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Furthermore, all treatments resulted in the downregulation of antioxidant metabolites such as glutathione, indicating that antibiotic-induced oxidative stress may surpass the bacterias antioxidant defense capability