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The hippocampi were carefully isolated and plated onto porous membrane inserts (Millipore, PIC0M03050) in 6-well plates with prewarmed OHSC culture media containing 50% MEM, 25% basal medium eagle (BME), 25% heat-inactivated horse serum, 2 mM glutamine (GlutaMAX-I), 1% antibiotic-antimycotic, 0.5 mM ascorbic acid, 0.05% insulin, 26.6 mM HEPES, 0.65% glucose, and 2.5 mM MgSO 4

These were used to plot concentration-response curves, which were analysed using a three-parameter logistic equation

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Nimchinsky et al., 2001