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Redox control of protein tyrosine phosphatases and mitogen-activated protein kinases in plants

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To further establish that R4VPL3-1 induced ferroptotic cell death and test for potential involvement of additional cell death pathways that may mediate R4VPL3-1-induced cell death, we pre-treated A375R melanoma cells either with DMSO (control), or with Z-VAD-FMK (pan-caspase inhibitor), chloroquine (lysosomal inhibitor), or Necrostatin (a necroptosis inhibitor), or Ferr-1 (ferroptosis inhibitor), and subsequently treated the cells with 3M R4VPL3-1

The effect of cardiolipin side chain composition on cytochrome c protein conformation and peroxidase activity

13 C-NMR (100 MHz, CDCl 3 ) ppm = 170.7, 156.5, 149.7, 146.1, 142.7, 142.2, 133.4, 124.2, 119.8, 115.0, 112.4, 109.9, 39.7, 35.5

The Matrigel-cell mixture was then implanted onto the chorioallantoic membrane (CAM) of the eggs on embryonic day 8 (EDD8)