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Physiological Effects of Selank and Its Fragments

Krukowski et al., 2018)

reduced levels of RhopH2 and RhopH3 resulted in parasites stalling about midway through their lifecycle, which is consistent with when NPPs are active (1536 h after invasion)

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after reduction and alkylation using DTT and IAA, the proteins were centrifuged on a 30 kDa cutoff filter device (Sartorius), washed twice with UA buffer (8 M urea in 0.1 M Tris/HCl pH 8.5) and twice with 50 mM ammoniumbicarbonate