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IGF-1 DES has an ultra-short half-life of about 20 to 30 minutes but approximately 10 times the potency at receptor sites compared to native IGF-1

Research trends in medical sciences

& Bainbridge, C

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metabolite Ac-LKKTE identified as potentially more active than parent compound Paradoxical finding: Both peptides show biological effects persisting hours to days after administration despite rapid plasma clearance Possible explanations: Tissue retention, active metabolites, or persistent signaling cascade activation Excretion Pathways Limited data exists on excretion for both peptides[22]: Likely renal elimination of peptide fragments Hepatic metabolism may contribute to clearance No accumulation detected in chronic dosing studies (animal models) Combined excretion kinetics have not been characterized in any species The disconnect between short plasma half-lives and prolonged biological effects represents a key area requiring mechanistic clarification for both individual peptides and their combination

To lyse brain cell membranes, homogenates were mixed with vortex for 1 min on ice three times with 10 min intervals