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Dried samples were dissolved in 20 l 0.1% formic acid, sonicated for 30 s and centrifuged for 10 min at 16,100 g

An enzymatic method was used to evaluate total bile acids in the serum and liver tissue while a targeted UPLC/MS method was also employed to evaluate select hepatic bile acids

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BrdU incorporation was analysed by Biozero fluorescence microscopy (Keyence)