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However, for long-term storage (months or years), you should store the lyophilized vials in a freezer, ideally below -20C

Bone physiological microenvironment and healing mechanism: Basis for future bone-tissue engineering scaffolds

The aqueous phase was adjusted to pH 10 with ammonium hydroxide and extracted with chloroform (5 ml 100 ml)

M.ReggianiF.et al (2017)

Cell Res 26 , 10211032 (2016)

Practical topics emphasized in the program include how to document working solution preparation, how to track concentration assumptions across serial dilutions, and how to align solvent choices with assay matrices to reduce unintended interference