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The mobile phase consisted of 0.1% formic acid in water (A) and methanol (B), with a gradient elution: 2% B to 30% B over 30 min, then to 60% B in 8 min, to 100% B in 2 min (hold 5 min), and re-equilibration to initial conditions in 1 min

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In severe cases, brown lesions can develop on nodes and internodes, sometimes merging to cause stalk rot and plant lodging ( 4.3.12 Rough dwarf disease of maize A severe ailment known as maize rough dwarf disease (MRDD) significantly lowers maize output

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1 and 2 (mutation sites) and 3 (glutathionylation sites)