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Under normal conditions, its lifespan ranges from a few microseconds to a few milliseconds (Glin 2020

Nuclei were pelleted by centrifugation (650 g, 5 min), supernatant was discarded and the pellet was resuspended in 100 l NUN1 buffer containing 20 mM Tris-HCL (pH = 7.5), 75 mM NaCl, 0.5 mM EGTA, 50 % glycerol, 1x protease and 1x phosphatase inhibitors

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28: The lancet 344 :796-798,1994 29: Nutrition reviews 54 :1-30,1996 30: clinical science 91 :575-582, 1996 31: Clinical science 91 :575-582, 1996 32: Hong SY, Gil HW, Yang JO, Lee EY, Kim HK, Kim SH, Chung YH, Hwang SK, Lee ZW (2005)

In high fat and high sugar model of NAFLD, cassia glycosides can obviously improve liver function and regulate blood lipids when compared with the commonly used drug polyene phosphatidylcholine in clinic, which is related with the inhibition of SREBP-1c expression in liver [112]

Cholesterol accumulation can also impair the function of innate immune system cells, such as natural killer (NK) cellscytotoxic lymphocytes of the innate immune system that recognize and eliminate stressed, transformed, or infected cells without prior sensitizationby reducing their cytotoxic capacity and increasing the risk of HCC in the context of MASLD [35, 36]