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Exploring the mechanisms underlying effects of oxygenated polycyclic aromatic hydrocarbons exposure on inflammatory bowel disease

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Hydrolyzed Ceramide : Reinforces the skin's natural barrier and prevents moisture loss

An aliquot of 20 l of the third-round virus mixture was used to infect 500 ml (SFM900-II medium with 5% FBS, Thermo Fisher) of Sf9 cells (1.5 10 6 /ml) for protein expression in an orbital shaker (Shanghai Zhichu Instrument) at 27 C and 200 rpm

These metal chelators bind to Mg 2+ and, thus, prevent cleavage of the purified protein by contaminating metalloproteases