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first, through the generation of reactive oxygen species (ROS) as a pro-oxidant involving superoxide anions (O 2 -), hydroperoxides (HO), singlet oxygen ( 1 O 2 ) and hydrogen peroxide (H 2 O 2 ) and second, by natural occurring antioxidants capacity destruction (Flora, 2002)

observing the depletion of H 2 O 2 in accordance with a drop in absorbance at 240 nm was used to measure CAT activity in the supernatant

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Potential Long-Term Risks Although AOD-9604 does not increase IGF-1 levels, there are concerns about its long-term metabolic effects, including: Unknown impact on fat metabolism regulation Since AOD-9604 alters fat-burning pathways, researchers are still investigating whether prolonged use could affect natural fat metabolism