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p21 positive cells detected by immunofluorescence method in mouse skin Paraffin-embedded mouse skins were immunolabelled with antibody to p21 (rat anti-mouse from AbCam), and immunofluorescence performed as we describe previously 64

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Figure 8 3.5 Chronic fatigue syndrome (CFS) A growing body of clinical evidence shows that the gut microbiota plays a critical role in CFS, with consistently observed reductions in butyrate-producing bacteria and enrichment of Enterobacteriaceae , together with frequent depletion of anti-inflammatory taxa such as Faecalibacterium (Knig et al., 2021

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No dose-escalation or maximum tolerated dose studies have been conducted, limiting understanding of safety at higher doses

Treating cells with cumene hydroperoxide was shown to enhance eryptosis, whereas the antioxidant NAC mitigated this process by enhancing RBC deformability and reducing levels of ROS, calcium, and PS exposure, and demonstrated that oxidative stress plays a crucial role in eryptosis, with pro-oxidants promoting and antioxidants limiting eryptotic processes [413]