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Membranes were blocked with 5% skim milk in TBST (0.1% Tween-20) prior to overnight incubation (4 C) with specific primary antibodies: CCR1 (1:1000, Origene, TA374392, MD, USA), TNF- (1:500, Bioworld, BS1857, Nanjing, China), IL-1 (1:1000, Abcam, ab283818, Cambridge, UK), ZO-1 (1:1000, Invitrogen, 33-9100, CA, USA), Occludin (1:1000, Invitrogen, 33-1500, CA, USA), UPK3 (1:1000, PROGEN, 690108S, Heidelberg, Germany), FOXO1 (1:1000, Proteintech, 18592-1-AP, Wuhan, China), p-FOXO1 (1:1000, Affinity, AF3416, Changzhou, China), PPAR (1:1000, Proteintech, 66826-1-Ig, Wuhan, China), PPAR (1:1000, Proteintech, 81490-5-RR, Wuhan, China), PPAR (1:1000, Proteintech, 60193-1-Ig, Wuhan, China), GAPDH (1:1000, CST, 2118S, MA, USA)

Hence, the facilitation of glutaminolysis leads to the accumulation of excess ammonia within cells, and a high concentration of ammonia is a potent inducer of autophagy 167 (Fig

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15 , R29 (2014)