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The relative mRNA levels of each target gene were estimated using the 2 Ct method, with the 18 S housekeeping gene serving as a reference

When MT2 reacted with AJO or ALL, the buffer was exchanged, effectively removing the dissociated Zn(II) from the sample

Nat Methods (2009) 6(2):1616

Yet, treatment of disease with antioxidant approaches has nearly uniformly failed (19, 20), perhaps partly because oxidative stress is often compartmentalized or driven by multiple nonspecific (chemical) and specific (enzymatic) pathways

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