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The kinetic data, which is presented as Lineweaver-Burk double reciprocal plots, revealed four linear relationships between 1/V and 1/[S], with varying slopes that intersect along the horizontal axis (Supplementary Fig

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Recent research suggests that metformin may also alter the gut microbiota, through the protection of the intestinal barrier, promotion of SCFAs producing bacteria growth, regulation of bile acids plasmatic levels and their excretion, and direct regulation of growth of bacteria with a positive effect on glucose homeostasis (eg., Akkermansia muciniphila ) (Zhang and Hu, 2020)

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