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These data integrate with a broader framework in which nervecancer interactions remodel tissue compartments, influence stromal states, and modulate immune surveillance ( This mini review synthesizes current knowledge on NGF/TrkA and BDNF/TrkB axes in osteosarcoma with emphasis on neurotrophin-driven remodeling of antitumor immunity

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The protein concentration was measured with BCA protein assay kit (Beyotime Institute of Biotechnology, Shanghai, China)