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Fourier transform infrared spectroscopy were obtained from a Nicolet iS20 Fourier transform infrared spectrometer (Thermo Scientific, USA)

From the standard curve values, the straight-line equation was calculated and the absorbance values of analyzed samples were converted into mg/dL

Lee et al., 2003

2018;314:H65968

Correct Coding: ICD-10 Code 1: M81.0 Age-related Osteoporosis without current fracture (Primary diagnosis) ICD-10 Code 2: E55.9 Vitamin D Deficiency, Unspecified (Secondary diagnosis) CPT Code: 82306 Vitamin D

After isolation of the target DNAprotein complexes, Proteinase K was added to degrade the protein components within the complexes, and the purified DNA products were recovered