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According to the above-mentioned results that Fusion 6 with L207A/L214A lost the cleavage activity completely, here we further replaced L207 with its isomer Ile to construct Fusion 9, trying to find a suitable substitution to avoid the alternative hydrolysis at L207

Quick Facts: GHK-Cu Peptide Definition: A naturally occurring copper-binding tripeptide composed of glycine, histidine, and lysine, complexed with a copper(II) ion