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Mood Changes and Irritability Low Vitamin D levels have been linked to depressive symptoms

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Studies consistently show 10 to 30 percent of metformin users develop low b12 levels over years of use

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Control groups of mice were maintained on LD or WD and given sham (10 mL/kg body weight sterile saline) injections throughout the study (termed LD/LD-V, or LD control, and WD/WD-V, or WD control, groups

When imputing missing data only, the underlying assumption is that body weight would be unaffected by discontinuation for trial participants lost to follow up