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In some embodiments, transfer vehicle variability between lots will be less than 15%, less than 10% or less than 5%

Falling asleep faster and sleeping more deeply are the earliest reported effects

A starter cultured was prepared by inoculating 10 ml of Terrific Broth (Fisher BioReagents) supplemented with 50 g ml 1 kanamycin sulfate (Fisher BioReagents) in a bacterial tube (Corning Falcon) and incubated in an Innova-44 Incubator-Shaker (New Brunswick) at 220 rpm overnight at 30 C (for FGF2 or TGF3) or 37 C (for NRG1)