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The samples were centrifuged, and the supernatant was collected and deproteinized using trichloroacetic acid at a final concentration of 20%

Aging unconventionally: T cells, inkt cells, and mait cells in aging

A Mitochondrial Metabolism Peptide MOTS-c (short for mitochondrial open-reading-frame of the 12S rRNA type-c) is a small peptide made of 16 amino acids, originally discovered in 2015

The ghrelin-induced ghrelin receptor activity was measured by NanoBiT-G-protein dissociation assay using a chimeric Gio protein according to the previous publication except for replacement Gqo with Gio 54