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Figure 1 During cartilage injury, macrophages play a crucial role

2.9 Lactate dehydrogenase (LDH) assay Briefly, 50 L from the apical chamber of GDC-treated monolayers were transferred to a 96-well flat-bottom plate, and mixed with equal volume of LDH Cytotoxicity Assay reaction mixture

After rinsing with PBS, nuclear staining was performed using DAPI (0.2 g/mL) and cells were analyzed by fluorescence microscopy (DMI4000B, Leica, Wetzlar, Germany) with 20x objective lens

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doi: 10.1016/j.cell.2007.04.040 150 LanzaM.FilipponeA.ArdizzoneA.CasiliG.PaternitiI.EspositoE.et al

7 / 12 Choose L-ascorbic Acid This form of topical vitamin C is the most active and the most researched