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Generally, were not able to accommodate one-off requests, but we will reach out to our partners at Flex and see if we can accommodate your request

Preparation of mRNA-LNPs mRNA encoding the Spike gene, EGFP , or Akaluc gene was synthesized by using T7 RiboMAX RNA synthesis kit (Promega) in the presence of 4 mM CleanCap AG (TriLink BioTechnologies), 5 mM 1-methylpseudouridine (Fujifilm), and 5 mM each of ATP, GTP, and CTP

Nicotinamide N-methyltransferase (NNMT) is a cytosolic enzyme that catalyzes the methylation of nicotinamide using S-adenosylmethionine (SAM) as a methyl donor, producing 1-methylnicotinamide (1-MNA) and S-adenosylhomocysteine (SAH)

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The cover slips were treated with poly-L-lysin solution (Sigma-Aldrich P8920) at RT for 30 min