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Acrolein is formed endogenously from polyamines by copper-dependent amine oxidases (plasma/serum amine oxidase, diamine oxidase, and semicarbazide-sensitive amine oxidase) and by FAD-dependent polyamine oxidases, monoamine oxidase, and spermine/polyamine oxidase [53]

Taken together these results indicate Cu 3 -TC-Py mediates oxidative DNA cleavage within the minor groove using a Fenton / Haber-Weiss type catalytic cycle and further supports the minor groove binding preference identified in earlier fluorescence melting experiments

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