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Supplementary Table 4 (download XLSX Muscle tissue RNA-seq data from day 2 for the experiment shown in Extended Data Fig

Enzyme-linked immunosorbent assay To detect binding of complement components or plasminogen, microtiter plates (Nunc MaxiSorp, Thermo Fisher Scientific) were coated with 100 l of purified His 6 -tagged proteins (5 g/ml) or BSA (5 g/ml) in PBS at 4 C overnight 74

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Rat full-thickness wounds (6mm diameter, 13-day study) treated with GHK showed 64.5% reduction in wound size compared to 45.6% for vehicle-treated and 28.2% for control group, accompanied by significantly lower TNF- and elastin-degrading MMPs