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Protein was expressed with suspension-adapted HEK293F cells by infecting the virus at a ratio of 1:10 (virus:cell, v/v)

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A method of eliciting an immune response in a subject in need thereof, comprising administering to the subject, optionally intramuscularly, intranasally, intravenously, subcutaneously, or intradermally, a prophylactically effective amount of the vaccine of claim 76 or 77

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