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Oxidative stress results from the excessive production of free radicals and oxidants within cells, surpassing the cells antioxidant defenses and leading to oxidative damage that hinders cellular proliferation 46

The cell culture medium was removed, and the cells were washed twice with PBS to remove residual medium and non-adherent cells

Roos RAC (2010) Huntingtons disease: a clinical review

doi: 10.1093/jxb/eru039 4 AyN.IrmlerK.FischerA.UhlemannR.ReuterG.HumbeckK

Vermeidung von oxidativem Stress durch eine Ernhrung reich an Antioxidantien (frisches, regionales, saisonales Obst, Gemse, Beeren, Pilze, Gewrze, Kaffee und Tee)

Since IRGQ affects the interaction between the hATG8s and the autophagy initiation machinery, we tested whether IRGQ assists in GABARAPL2 or LC3B lipidation