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Briefly, 5 l of hemolyzed blood was diluted in 1 ml of buffer potassium phosphate 0.05 M pH 7.0 with EDTA 0.1 mM, and 10 l of H 2 O 2 1 M according to Beers and Sizer 49

These good old days of simple biology are long gone in the wake of new findings, and emerging concept of mitochondrial ROS production is becoming more and more complex as new elements are discovered

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