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Just remember to make sure that you bring up your questions during the consultation because once the procedure has started, there is no turning back.

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The supernatant of G422 cells after treatment of BEAGA/ApoE-PDHD plus LFUS irradiation mentioned above was collected and dried up

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Then, the astrocytes were stained with goat anti-rabbit secondary antibody (1:100, Invitrogen, USA) and DAPI (1:1000, Sigma-Aldrich, USA)