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Briefly, cells were cultured in CDSS-containing media for 8 weeks to generate castration-resistant LNCaP-CR cells, which were then cultured in CDSS medium containing Enza (20 M) for 2 weeks to derive LNCaP-C/Enza-R cells (supplementary Fig

These methods allow the vitamin to be absorbed directly into the bloodstream

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While the trial failed to demonstrate a significant slowing in disease progression with either dose, the findings suggested that a subgroup of patients with early ALS and moderate disease progression could potentially benefit from the higher dose

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