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Polyamine metabolism in sunflower plants under long-term cadmium or copper stress

For purification, 2030 g of cells were resuspended in lysis buffer comprised of 2xTBS (40 mM Tris-HCl pH 8.0, 300 mM NaCl) and supplemented with 10 mM benzamidine, 10 mM 6-aminocaproic acid and 1 mM PMSF

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Second, the underlying pathophysiology of ASD remains poorly understood, and the specific contribution of the MGBA warrants further exploration