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In traditional preparations, endogenous bacteria from cabbage initiate fermentation in an anaerobic brine environment, converting plant-derived carbohydrates into lactic acid along with carbon dioxide, acetic acid, and various bioactive compounds (National-Research-Council, 1992

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RT-PCR was used to establish accurate cycling for barcode incorporation and final library amplification, avoiding over- or under-cycling libraries, which could skew transcript counts during sequencing